# 蛋白质理化性质
## 跨膜结构域分析：DeepTMHMM预测
biolib run DTU/DeepTMHMM --fasta /home/xia-lab/wutx/00work/01.gene_family/geneFamily20200803/62.SPL_tree/00.alignment/Ft.SPL.pep.fasta

## 蛋白质等电点和分子量计算
- 计算SPL基因的分子量和等电点
Rscript  scripts/fasta_pI_mw.R Ft.SPL.pep.fasta  Ft.SPL.pep.fasta.pI_mw

- 生成SPL基因汇总统计表格（可放文章附件）
perl scripts/GeneFamily_stat.pl ../00.genome/Ft_final.gff3   Ft.SPL.pep.fasta.pI_mw > Ft.SPL.stat

## 亚细胞定位
- Plant-mPLoc预测
python scripts/get_Plant_mPLoc_out.py --input home/wutx/00.work/01.gene_family/geneFamily20200803/62.SPL_tree/00.alignment/ziji_danbaizhi_tezheng/Ft.SPL.pep.fasta --output Ft.SPL_out.txt

- WoLF PSORT 预测
python scripts/wolfpsort_batch.py --input ../../00.alignment/Ft.SPL.pep.fasta --output Ft.SPL_wolf_out.txt