45 lines
1.4 KiB
YAML
Executable File
45 lines
1.4 KiB
YAML
Executable File
# Functional Annotation Skill Configuration
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# 功能注释技能 - 使用 eggNOG-mapper 进行蛋白功能注释
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skill:
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name: annotation
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version: "1.0.0"
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description: "功能注释分析 - eggNOG-mapper"
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author: "bbav20311-team"
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input:
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protein_fasta: null # 必需: 蛋白序列FASTA文件
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parameters:
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emapper:
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# eggNOG-mapper 主要参数
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mode: "diamond" # 搜索模式: diamond/mmseqs/hmmer
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evalue: 0.001 # E-value阈值
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score: 60 # 最小bit score
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query_cover: 20 # 最小query覆盖度(%)
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subject_cover: 20 # 最小subject覆盖度(%)
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tax_scope: "auto" # 分类范围: auto/bact/arch/euk/viruses
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go_evidence: "non-electronic" # GO证据: all/non-electronic/experimental
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target_orthologs: "all" # 目标直系同源: all/one2one/one2many
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# 数据库路径 (可选，默认使用 ~/.eggnog-mapper/)
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data_dir: null # eggNOG 数据库目录，如 /path/to/eggnog_db
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# 性能参数
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cpu: 8
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block_size: 5 # Diamond block size (内存控制)
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output:
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format: ["annotations", "seed_orthologs", "pfam", "cog", "kegg"]
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report_template: "templates/report.md.j2"
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include_reproducibility: true
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dependencies:
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conda:
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channels:
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- bioconda
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- conda-forge
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packages:
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- eggnog-mapper=2.1.12
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- diamond=2.1.8
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- biopython=1.81
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- pandas=2.0.0
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