admin/PlantAI-dev
1
0
Fork 0
Code Issues Pull Requests Actions Packages Projects Releases 1 Wiki Activity
Files
master
PlantAI-dev/tools/interproscan-5.78-109.0/bin/prosite/ps_scan_README
T
potaser f745ee9d7e Initial commit: PlantAI-dev
2026-08-03 18:42:47 +08:00

252 lines
9.9 KiB
Plaintext
Executable File
Raw Permalink Blame History

ps_scan documentation
=====================
Reference:
Alexandre Gattiker, Elisabeth Gasteiger, Amos Bairoch.
ScanProsite: a reference implementation of a PROSITE scanning tool.
Applied Bioinformatics 2002:1(2) 107-108.
Contact: prosite@expasy.org
ps_scan is a perl program used to scan one or several patterns, rules and/or
profiles from PROSITE against one or several protein sequnces in Swiss-Prot or
FASTA format. It requires two compiled external programs from the PFTOOLS
package : "pfscan" used to scan a sequence against a profile library and
"psa2msa" which is necessary for the "-o msa" output format only.
Authors:
Alexandre Gattiker
Edouard de Castro; E-mail: ecastro@sib.swiss
Elisabeth Gasteiger
Installation
------------
Download a binary package for your platform from
https://ftp.expasy.org/databases/prosite/ps_scan/ps_scan_<platform>.tar.gz
or the source packages from
https://ftp.vital-it.ch/pub/software/unix/pftools/ or https://github.com/sib-swiss/pftools3
in which case you will need gcc or a compatible fortran compiler to compile the
pftools sources, see https://ftp.expasy.org/databases/prosite/README.pftools.
You may need to edit the ps_scan.pl to provide absolute paths to the directory
where you have installed the pfscan and psa2msa executables, unless you have
stored them in a directory in your PATH.
A local copy of the PROSITE database is also needed. You may download it
in a single file from
https://ftp.expasy.org/databases/prosite/prosite.dat
Usage
-----
perl ps_scan.pl [options] sequence-file(s)
ps_scan version $VERSION options:
-h : this help screen
Input/Output:
-e : specify the ID or AC of an entry in sequence-file
-o : specify an output format : $formats_string
-d : specify a prosite.dat file
-p : specify a pattern or the AC of a prosite motif
-f : specify a motif AC to scan against together with all its
releated post-processing motifs (but show only specified
motif hits)
Selection:
-r : do not scan profiles
-m : only scan profiles
-s : skip frequently matching (unspecific) patterns and profiles
-l : cut-off level for profiles (default : 0)
Pattern match mode:
-x : specify maximum number of accepted matches of X's in sequence
(default=0)
-g : Turn greediness off
-v : Turn overlaps off
-i : Allow included matches
The sequence-file may be in Swiss-Prot or FASTA format.
If no PROSITE file is submitted, it will be searched in the paths
$PROSITE/prosite.dat and $SPROT/prosite/prosite.dat.
There may be several -d, -p and -q arguments.
Pfsearch options:
-w pfsearch : Compares a query profile against a protein sequence library.
A profile file must be specified with option -d.
$progname -w pfsearch [-C cutoff] [-R] -d profile-file seq-library-file(s)
-R: use raw scores rather than normalized scores for match selection
-C=# : Cut-off value. Reports only match score higher than the specified
parameter.
An integer argument is interpreted as a raw score value,
a decimal argument as a normalized score value. An integer value forces
option -R.
Output formats
--------------
An output format may be specified with the -o option. Here are the available
formats, with an example output with a profile :
-o scan : default ps_scan output format.
YAHD_ECOLI : PS50088 ANK_REPEAT Ankyrin repeat profile.
12 - 37 -------LAAQQGDIDKVKTCLALGVDINTCDR L=-1
38 - 70 QGKTAITLASLYQQYACVQALIDAGADINKQDH L=0
138 - 175 VGWTPLLEAIVLNDGGikqqaIVQLLLEHGASPHLTDK L=0
176 - 201 YGKTPLELARERGFEEIAQLLIAAGA------- L=0
-o fasta : matching sequences in FASTA format.
>YAHD_ECOLI/12-37 : PS50088 ANK_REPEAT L=-1 Ankyrin repeat profile.
LAAQQGDIDKVKTCLALGVDINTCDR
>YAHD_ECOLI/38-70 : PS50088 ANK_REPEAT L=0 Ankyrin repeat profile.
QGKTAITLASLYQQYACVQALIDAGADINKQDH
>YAHD_ECOLI/138-175 : PS50088 ANK_REPEAT L=0 Ankyrin repeat profile.
VGWTPLLEAIVLNDGGIKQQAIVQLLLEHGASPHLTDK
>YAHD_ECOLI/176-201 : PS50088 ANK_REPEAT L=0 Ankyrin repeat profile.
YGKTPLELARERGFEEIAQLLIAAGA
-o psa : profile-to-sequence-alignments with insertions in lowercase and
deletions marked as dashes.
>YAHD_ECOLI/12-37 : PS50088 ANK_REPEAT L=-1 Ankyrin repeat profile.
-------LAAQQGDIDKVKTCLALGVDINTCDR
>YAHD_ECOLI/38-70 : PS50088 ANK_REPEAT L=0 Ankyrin repeat profile.
QGKTAITLASLYQQYACVQALIDAGADINKQDH
>YAHD_ECOLI/138-175 : PS50088 ANK_REPEAT L=0 Ankyrin repeat profile.
VGWTPLLEAIVLNDGGikqqaIVQLLLEHGASPHLTDK
>YAHD_ECOLI/176-201 : PS50088 ANK_REPEAT L=0 Ankyrin repeat profile.
YGKTPLELARERGFEEIAQLLIAAGA-------
-o msa : multiple sequence alignment of the matches in each sequence, built
from the psa format with gaps marked as dots.
>YAHD_ECOLI/12-37 : PS50088 ANK_REPEAT L=-1 Ankyrin repeat profile.
-------LAAQQGDID.....KVKTCLALGVDINTCDR
>YAHD_ECOLI/38-70 : PS50088 ANK_REPEAT L=0 Ankyrin repeat profile.
QGKTAITLASLYQQYA.....CVQALIDAGADINKQDH
>YAHD_ECOLI/138-175 : PS50088 ANK_REPEAT L=0 Ankyrin repeat profile.
VGWTPLLEAIVLNDGGikqqaIVQLLLEHGASPHLTDK
>YAHD_ECOLI/176-201 : PS50088 ANK_REPEAT L=0 Ankyrin repeat profile.
YGKTPLELARERGFEE.....IAQLLIAAGA-------
-o pff : tabular format listing bounding positions on the sequence and the
profile, the raw and normalized profile score, and the cut-off level.
YAHD_ECOLI 12 37 ANK_REPEAT 8 -1 196 6.653 -1
YAHD_ECOLI 38 70 ANK_REPEAT 1 -1 351 10.793 0
YAHD_ECOLI 138 175 ANK_REPEAT 1 -1 288 9.110 0
YAHD_ECOLI 176 201 ANK_REPEAT 1 -8 343 10.579 0
Pattern and rule matches are reported in the same formats, but cut-off levels
are not available. In scan, psa and msa output formats, amino acids matching an
"x" in the pattern are reported in lowercase. In pff format only the first
three columns are available. The "-o matchlist" output format is specific to
patterns and not meant for public use.
Profile and pattern matching parameters
---------------------------------------
Several parameters can be fine-tuned.
- profile cut-off levels (-l option)
Most profiles contain several cut-off levels. The level 0 cut-off is the
trusted cut-off for positive matches, but a level -1 is usually defined above
which a match is potential, especially if there are other matches in the
sequence with the profile. By default, pfscan is run with the cut-off
level 0, so that only trusted matches are reported. To retrieve potential
(weak) matches as well, run ps_scan with the option "-l -1". Weak matches
are then reported with the indication "L=-1".
- skip frequently matching patterns (-s option)
Some PROSITE entries such as those describing commonly found post-
translational modifications (a typical example is N-glycosylation) are found
in the majority of known protein sequences. While it is generally useful to
note their presence, some programs may want, in some cases, to ignore those
entries, which contain the line "CC /SKIP-FLAG=TRUE;".
Pattern match mode:
- pattern greediness off (-g option)
A pattern-matching engine is said to be "greedy" if it tries to extend at
most variable-length pattern elements. The sequence "ABCDC" and the pattern
"A-x(1,3)-C" will produce the match "ABCDC" with a greedy engine, and the
match "ABC" with a non-greedy one. By default, PROSITE is scanned in greedy
mode, unless the -g option is set.
- overlaps (-v option) and included matches (-i option)
Some patterns may produce distinct but overlapping matches on a given
sequence. Additionally, if the pattern contains variable-length elements,
some of these matches may be completely included in another one. Different
combinations of -g, -v and -i options may produce differences in match count
and match positions with patterns that contain variable-length elements. An
engine which allows overlaps should be greedy in order to reduce the number
of multiple hits which are almost entirely overlapping except at the
extremities.
- treatment of X characters in sequences (-x option)
The PROSITE syntax describes how to treat ambiguities in the pattern, but not
how to handle ambiguities in the sequence. In rare sequences in Swiss-Prot
and other databases, the characters B and Z are used according to IUBMB
nomenclature when a residue may be either Asp or Asn, or Glu or Gln,
respectively. The ps_scan program will produce a match if the sequence has a
"B" and the pattern allows either a "D" or a "N", or both (and similarly for
Z).
Whether the character X should be allowed to match any position of a pattern
is more controversial. It is generally useful to accept a single pattern
position to match X (unless that pattern position is an X itself, in which
case we can accept more). The maximum number of X characters which are
allowed to match a non-X position in a pattern can be specified with the -x
option. The default value is 0.
Examples
--------
examples :
scan 1 sequence with 1 pattern
perl ps_scan.pl -p PS00123 seq.dat
perl ps_scan.pl -p "P-S-[QW]" seq.dat
scan 1 sequence with all prosite, including profiles
perl ps_scan.pl seq.dat
scan 2 profiles or patterns against swiss-prot
perl ps_scan.pl -p PS00123 -p PS00124 sprot.dat
scan all patterns and rules against swiss-prot
perl ps_scan.pl -r sprot.dat
scan a profile file against a sequence file
perl ps_scan.pl -d profile.prf sprot.dat
License
-------
The ps_scan program and the Prosite.pm module are Copyright (C) 2001-2006,
Swiss Institute of Bioinformatics. They are released under the
terms of the GNU General Public License, available at
http://www.gnu.org/copyleft/gpl.html.
This program is free software; you can redistribute it and/or modify it under
the terms of the GNU General Public License as published by the Free Software
Foundation; either version 2 of the License, or (at your option) any later
version.
This program is distributed in the hope that it will be useful, but WITHOUT ANY
WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A
PARTICULAR PURPOSE. See the GNU General Public License for more details.
Reference in New Issue View Git Blame Copy Permalink