diff --git a/bioflow_service/app/services/bioflow/extension_exec.py b/bioflow_service/app/services/bioflow/extension_exec.py
index 460250c..608f0a9 100644
--- a/bioflow_service/app/services/bioflow/extension_exec.py
+++ b/bioflow_service/app/services/bioflow/extension_exec.py
@@ -15,6 +15,7 @@ from __future__ import annotations
 
 import asyncio
 import logging
+import os
 import shlex
 from dataclasses import dataclass, field
 from pathlib import Path
@@ -261,8 +262,22 @@ def _default_exec_config() -> ExtensionExecConfig:
 
 
 def _resolve_extension_entry_dir() -> str:
-    """宿主入口脚本目录 = repo 根 bioflow_kernel/cli(按 __file__ 推导,零硬编码)。"""
+    """宿主入口脚本目录 = 部署树 bioflow_kernel/cli(env 优先 + 多候选推导)。
+
+    2026-09-15 实测:parents[4] 推导在 175 仓库布局成立,176 部署树路径深度
+    不同 → 找到不存在位置 → 试运行报「入口脚本缺失」。修复:
+    1. BIOFLOW_EXT_ENTRY_DIR env 显式指定(部署树配置)
+    2. 从 __file__ 向上逐级找 */bioflow_kernel/cli/run_extension.py
+    3. 兜底 parents[4] 推导
+    """
+    env_dir = os.environ.get("BIOFLOW_EXT_ENTRY_DIR", "").strip()
+    if env_dir and Path(env_dir).is_dir():
+        return env_dir
     here = Path(__file__).resolve()
+    for parent in [here] + list(here.parents):
+        candidate = parent / "bioflow_kernel" / "cli"
+        if (candidate / "run_extension.py").is_file():
+            return str(candidate)
     return str(here.parents[4] / "bioflow_kernel" / "cli")