19 lines
872 B
Markdown
Executable File
19 lines
872 B
Markdown
Executable File
# 蛋白质理化性质
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## 跨膜结构域分析：DeepTMHMM预测
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biolib run DTU/DeepTMHMM --fasta /home/xia-lab/wutx/00work/01.gene_family/geneFamily20200803/62.SPL_tree/00.alignment/Ft.SPL.pep.fasta
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## 蛋白质等电点和分子量计算
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- 计算SPL基因的分子量和等电点
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Rscript scripts/fasta_pI_mw.R Ft.SPL.pep.fasta Ft.SPL.pep.fasta.pI_mw
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- 生成SPL基因汇总统计表格（可放文章附件）
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perl scripts/GeneFamily_stat.pl ../00.genome/Ft_final.gff3 Ft.SPL.pep.fasta.pI_mw > Ft.SPL.stat
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## 亚细胞定位
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- Plant-mPLoc预测
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python scripts/get_Plant_mPLoc_out.py --input home/wutx/00.work/01.gene_family/geneFamily20200803/62.SPL_tree/00.alignment/ziji_danbaizhi_tezheng/Ft.SPL.pep.fasta --output Ft.SPL_out.txt
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- WoLF PSORT 预测
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python scripts/wolfpsort_batch.py --input ../../00.alignment/Ft.SPL.pep.fasta --output Ft.SPL_wolf_out.txt
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